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Information and registration links for Hands-on PGDIS Workshops:


PGDIS Workshop: May 21-23, 2012 Istanbul, Turkey

Session Title: HANDS-ON WORKSHOP ON PGD FOR GENETIC AND CHROMOSOMAL DISORDERS BY BLASTOCYST BIOPSY

Registration for the May 21-23, 2012 Workshop is Closed. You may reserve your spot for the next year Workshop, May 6-8, 2013, prior to International PGD Congress in Istanbul, May 9-11, 2013 - Class limit: 15 applicants

Schedule:

Monday, MAY 21

 
 
08.30 - 09.00 REGISTRATION
 
09.00 - 09.05 Opening of Workshop - Semra KAHRAMAN, PGDIS
 
09.05 - 09.35 Practical PGD - Anver KULIEV, PGDIS, Chicago, USA
 
09.35 - 10.05 Advantages and disadvantages of trophectoderm biopsy -
James MARSHALL, Sydney, Australia
 
10.05 - 10.35 DISCUSSION & COFFEE BREAK
 
10.35 - 13.10

Hands-on Applications of Biopsy under Supervision of Trainers
Participants will be divided into 3 groups, up to 6 in each for practical sessions.

- Zona Drilling Methods
- Blastomere Biopsy Techniques
- Trophectoderm Biopsy Techniques

 
13.10 - 14.20 LUNCH AND DISCUSSION
 

14.20 - 18.30

Each group will apply all the demonstrated techniques

 

Tuesday, MAY 22

 
 
09.00 - 09.20 Place of PGD in ART and Genetic Practices - Semra KAHRAMAN and Cumhur EKMEKCI, Istanbul, Turkey
 
09.20 - 09.40 Effectiveness of PGD for aneuploidy and translocations by FISH - Cagri BEYAZYUREK, Istanbul, Turkey
 
09.40 - 10.00

Molecular techniques in PGD: Testing for single gene disorders, HLA Typing, and 24 chromosome aneuploidy - Don LEIGH, Sydney, Australia

 
10.00 - 10.30 DISCUSSION AND COFFEE BREAK
 
10.30 - 13.30 Hands-on applications of trophectoderm biopsy by participants under supervision of trainers
 
13.30 - 14.30 LUNCH AND DISCUSSION
 
14.30 - 18.30

Hands-on Applications of FISH and PCR techniques under supervision of trainers

- Preparation, spreading and fixation of blastomere and trophectoderm cells

- In situ hybridization with different chromosome specific probes

- Analysis and Scoring of FISH signals

- 1st and 2nd Round PCR for PGD of single gene disorders and HLA typing

- Testing for 24 chromosomes by Microarray Technology

Participants will be divided into 3 groups, up to 6 in each for practical sessions according to their interests

 

Wednesday, MAY23

Interpreting FISH and PCR results
 
09.00 - 12.00

Analysis and scoring of FISH signals - supervised by Cagri BEYAZYUREK, Istanbul, Turkey

Analysis and interpretation of PCR results - supervised by Don LEIGH and James MARSHALL, Syndey, Austrralia

12.00 - 13.00 GENERAL DISCUSSION AND CLOSING
 
   
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