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Information and registration links for Hands-on PGDIS Workshops:


PGDIS Workshop: May 10-12, 2010 Istanbul, Turkey

Session Title: POST-CONGRESS HANDS-ON WORKSHOP ON PGD FOR GENETIC AND CHROMOSOMAL DISORDERS BY BLASTOCYST BIOPSY

Class limit: 15 applicants

ADVISORY: Registration for the PGDIS Genetic and Chromosomal Disorders by Blastocyst Biopsy workshop is CLOSED: Those who were not accommodated this year, may request rescheduling their participation for the Workshop next year, which will be held in Istanbul, dates to be announced in due course.

Schedule:

Monday, May 10

 
 
08.30 - 09.00 REGISTRATION
 
09.00 - 09.05 Opening of Workshop - Semra KAHRAMAN, PGDIS
 
09.05 - 09.35 Practical PGD - Anver KULIEV, PGDIS, Chicago, USA
 
09.35 - 10.05 Advantages and disadvantages of trophectoderm biopsy -
Maria TRAVERSA, Sydney, Australia
 
10.05 - 10.35 DISCUSSION & COFFEE BREAK
 
10.35 - 13.10

Hands-on Applications of Biopsy under Supervision of Trainers
Participants will be divided into 3 groups, up to 6 in each for practical sessions.

- Zona Drilling Methods
- Blastomere Biopsy Techniques
- Trophectoderm Biopsy Techniques

 
13.10 - 14.20 LUNCH AND DISCUSSION
 

14.20 - 18.30

Each group will apply all the demonstrated techniques

 

Tuesday, MAY 11

 
 
09.00 - 09.20 Clinical Aspects of PGD - Semra KAHRAMAN and Cumhur EKMEKCI, Istanbul, Turkey
 
09.20 - 09.40 Effectiveness of FISH for PGD for aneuploidy and translocations - Cagri BEYAZYUREK, Istanbul, Turkey
 
09.40 - 10.00

Molecular techniques in PGD of single gene disorders - Don LEIGH, Sydney, Australia

 
10.00 - 10.30 DISCUSSION AND COFFEE BREAK
 
10.30 - 13.30 Hands-on Applications of blastomere and trophectoderm biopsy by participants under supervision of trainers
 
13.30 - 14.30 LUNCH AND DISCUSSION
 
14.30 - 18.30

Hands-on Applications of FISH and PCR techniques under supervision of trainers

- Preparation, spreading and fixation of blastomere and trophectoderm cells

- In situ hybridization with different chromosome specific probes

- Analysis and Scoring of FISH signals

- 1st and 2nd Round PCR for PGD of single gene disorders

Participants will be divided into 3 groups, up to 6 in each for practical sessions according to their interests

 

Wednesday, MAY12

Interpreting FISH and PCR results
 
09.00 - 12.00

Analysis and scoring of FISH signals - supervised by Cagri BEYAZYUREK, Istanbul, Turkey

Analysis and interpretation of PCR results - supervised by Don LEIGH, Syndey, Austrralia

12.00 - 13.00 GENERAL DISCUSSION AND CLOSING
 
   
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