---

David Cram has a PhD from Monash University, Melbourne Director of Molecular Genetic Services, Monash (1998-2008) Currently Head of Genetics, Berry Genomics Corporation, Director of PGT of Children’s Hospital of Shanxi, and Director of Next Generation Solutions, Limited

Key Publications

1. Cram DS#, Song B, Trounson AO. Genotyping of Rhesus SCNT pluripotent stem cell lines. Nature, 2007; 450:E12-14.

2. Wang L, Cram DS#, Shen J, Wang X, Zhang J, Song Z, Xu G, Li N, Fan J, Wang S, Luo Y, Wang J, Yu L, Liu J, Yao Y. Validation of copy number variation sequencing for detecting chromosome imbalances in preimplantation embryos. Biology of Reproduction, 2014; 91:37.

3. Wang L, Shen J, Cram DS#, Ma M, Wang H, Zhang W, Fan J, Gao Z, Zhang L, Zhang J, Li Z, Xu M, Leigh D, Trounson AO, Liu J, Yao Y. Preselection and transfer of euploid non-carrier embryos in preimplantation genetic diagnosis cycles for reciprocal translocations. Fertility and Sterility, 2017; 108:620-627.

4. Liang D, Cram DS#, Tan H, Linpeng S, Liu Y, Sun H, Zhang Y, Tian F, Zhu H, Xu M, Wang H, Yu F, Wu L. Clinical utility of noninvasive prenatal testing for expanded chromosome disease syndromes. Genetics in Medicine, 2019; 126:1466-1474.

5. Cram DS#, Leigh D, Handyside A, Rechitsky L, Xu K, Harton G, Rubio C, Fragouli E, Kahraman S, Forman E, Katz-Jaffe M, Tempest H, Thornhill A, Strom C, Escudero T, Qiao J, Munne S, SimpsonJL, Kuliev A. PGDIS position statement on the transfer of mosaic embryos 2019.

6. Niclis J, Trounson AO, Dottori M, Ellisdon A, Bottomley S, Verlinsky Y, Cram DS#. Human embryonic stem cell models of Huntington’s Disease. Reproductive Biomedicine Online, 2009; 19:106-113.

7. Liang D, Peng Y, Lv W, Deng L, Zhang Y, Li H, Yang P, Zhang J, Song Z, Xu G, Cram DS#, Wu L. Copy number variation sequencing for comprehensive diagnosis of human chromosome disease syndromes. Journal of Molecular Diagnosis, 2014; 16:519-526.

8. Lv W, Wei X, Guo R, Liu Q, Zheng Y, Chang J, Bai T, Li H, Zhang J, Song Z, Cram DS#, Liang D, Wu L. Non-invasive prenatal testing for Wilson Disease using circulating Single Molecule Amplification and Re-sequencing Technology (cSMART). Clinical Chemistry, 2015; 61:172-181.

9. Li N, Wang L, Wang H, Ma M, Wang X. Li Y, Wang S, Zhang W, Zhang J, Cram DS#, Yao Y. The performance of whole genome amplification methods and next generation sequencing for pre-implantation genetic diagnosis of chromosomal abnormalities. Journal of Genetics and Genomics, 2015; 42:151-159.

10. Song Y, Zhou X, Huang S, Li X, Qi Q, Jiang Y, Liu Y, Zhang J, Xu M, Cram DS#, Liu J. Quantitation of fetal DNA fraction in maternal plasma using single molecule amplification and re-sequencing technology (cSMART). Clinica Chimica Acta, 2016; 456:151-156.

11. Chen Y, Liu Y, Wang B, Mao J, Wang T, Ye K, Ye Y, Cram DS#, Li H. Development and validation of of a novel genotyping assay for noninvasive prenatal diagnosis of hereditary hearing loss. Prenatal Diagnosis, 2016; 36:1233-1241.

12. Xu L, Mao A, Zhang J, Liu H, Gui B, Choy KW, Huang H, Yu Q, Zhang X, Chen M, Lin N, Chen L, Han J, Wang Y, Zhang M, Li X, He D, Lin Y, Zhang J, Cram DS#, Cao H. Long-molecule sequencing: A new approach for identification of clinically significant DNA variants in alpha and beta-thalassemia carriers. Journal of Molecular Diagnostics, 2020; 22:1087-1095.

13. Wang H, Jia Z, Mao A, Xu B, Wang S, Wang L, Liu S, Zhang H, Zhang X, Yu T, Mu T, Xu M, Cram DS#, Yao Y. Analysis of balanced reciprocal translocations in patients with subfertility using single- molecule optical mapping. Journal of Assisted Reproduction and Genetics, 2020; 37:509-516.

14. Rechitsky L, Kuliev A, San Ramon G, Tur-Kaspa I, Wang W, Wu X, Wang L, Leigh D, Cram DS#. Single molecule sequencing: a new approach for preimplantation testing and noninvasive diagnosis confirmation of fetal genotype. Journal of Molecular Diagnostics, 2020; 22:220-227.

15. Liu S, Song L, Cram DS, Xiong L, Wang K, Wu R, Liu J, Deng K, Jia B, Zhong M, Yang F. Traditional karyotyping vs copy number variation sequencing for detection of chromosomal abnormalities associated with spontaneous miscarriage. Ultrasound in Obstetrics and Gynecology, 2015 46:472-477.

16. Lv W, Li Z, Wei, X, Zhu H, Teng Y, Zhou M, Gong Y, Cram DS, Liang D, Han L, Wu L. Noninvasive fetal genotyping in pregnancies at risk for PKU using a comprehensive quantitative cSMART assay for PAH gene mutations: a clinical feasibility study. British Journal of Obstetrics and Gynaecology, 2019; 126:1466-1474.

17. Liu S, Wang H, Leigh D, Cram DS, Wang L, Yao Y. Third Generation Sequencing: any future opportunities. Journal of Assisted Reproduction and Genetics, 2020